Claudin 3 (Phospho-Tyr219) Antibody

Catalog Number:

11791

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O15551;
NCBI Gene#: 1365;
NCBI Protein#: NP_001297.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y(p)-V) derived from Human Claudin 3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.

Applications:

WB

Background:

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.

Peacock R.E., Genomics 46:443-449(1997).
Katahira J., J. Biol. Chem. 272:26652-26658(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF