C/EBP-α (Phospho-Thr230) Antibody

Catalog Number:

11788

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P49715;
NCBI Gene#: 1050;
NCBI Protein#: NP_004355.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 230 (P-P-T(p)-P-V) derived from Human C/EBP-α.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The Antibody detects endogenous levels of C/EBP-α only when phosphorylated at Thr230.

Applications:

WB

Background:

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113(1995)
Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379(1997)
SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF