4E-BP1 (Phospho-Thr70) Antibody

Catalog Number:

11781

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13541;
NCBI Gene#: 1978;
NCBI Protein#: NP_004086.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine70 (T-K-T(p)-P-P) derived from Human 4E-BP1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

Applications:

IHC

Background:

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Pause A., Nature 371:762-767(1994).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Haghighat A., EMBO J. 14:5701-5709(1995).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

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