Ephrin B1/B2/B3 (phospho Y324/Y311/Y318) Antibody

Catalog Number:

11780

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P98172/P52799/Q15768;
NCBI Gene#: 1947/1948/1949;
NCBI Protein#: NP_004420.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 324/311/318 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318.

Applications:

WB IHC

Background:

This gene encodes a member of the ephrin family. The encoded protein is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.

Beckmann M.P., EMBO J. 13:3757-3762(1994).
Davis S., Science 266:816-819(1994).
Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF