WWOX (Phospho-Tyr33) Antibody

Catalog Number:

11779

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q96KM3;
NCBI Gene#: 51741;
NCBI Protein#: NP_057457.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.

Applications:

WB IHC

Background:

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.

Bednarek A.K., Cancer Res. 60:2140-2145(2000).
Ried K., Hum. Mol. Genet. 9:1651-1663(2000).
Bednarek A.K., Cancer Res. 61:8068-8073(2001).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF