PYK2 (Phospho-Tyr579) Antibody

Catalog Number:

11776

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q14289;
NCBI Gene#: 2185;
NCBI Protein#: NP_004094.3.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 579(E-D-Y(p)-Y-K) derived from Human PYK2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.

Applications:

WB IHC

Background:

Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.

Lev S., Nature 376:737-745(1995).
Herzog H., Genomics 32:484-486(1996).
Sasaki H., J. Biol. Chem. 270:21206-21219(1995).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF