FGFR1/2 (Phospho-Tyr463/466) Antibody

Catalog Number:

11772

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P11362/P21802;
NCBI Gene#: 2260/2263;
NCBI Protein#: NP_075598.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 463/466(S-E-Y(p)-E-L) derived from Human FGFR1/2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of FGFR1/2 only when phosphorylated at tyrosine 463/466.

Applications:

WB

Background:

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation.

Itoh N., Biochem. Biophys. Res. Commun. 169:680-685(1990).
Dionne C.A., EMBO J. 9:2685-2692(1990).
Johnson D.E., Mol. Cell. Biol. 10:4728-4736(1990).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF