DDR1 (Phospho-Tyr513) Antibody

Catalog Number:

11770

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q08345;
NCBI Gene#: 780;
NCBI Protein#: NP_001189450.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 513 (K-K-Y(p)-V-R) derived from Human DDR1 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513.

Applications:

WB IHC

Background:

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI).

di Marco E., J. Biol. Chem. 268:24290-24295(1993).
Johnson J.D., Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681(1993).
Laval S., Cell Growth Differ. 5:1173-1183(1994).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF