BL-CAM (Phospho-Tyr807) Antibody

Catalog Number:

11765

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P20273;
NCBI Gene#: 933;
NCBI Protein#: NP_001762.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 807 (G-D-Y(p)-E-N) derived from Human BL-CAM.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of BL-CAM only when phosphorylated at tyrosine 807.

Applications:

WB

Background:

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Wilson G.L., J. Exp. Med. 173-137-146(1991).
Wilson G.L., J. Immunol. 150:5013-5024(1993).
Stamenkovic I., Nature 345-74-77(1990).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF