IRF-3 (Phospho-Ser386) Antibody

Catalog Number:

11760

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q14653;
NCBI Gene#: 3661;
NCBI Protein#: NP_001184052.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 386(A-S-S(p)-L-E) derived from Human IRF-3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 386.

Applications:

WB

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Bellingham J., Ann. Hum. Genet. 62:231-234(1998).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF