TIE2 (Phospho-Tyr992) Antibody

Catalog Number:

11756

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q02763;
NCBI Gene#: 7010;
NCBI Protein#: NP_000450.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y(p)-V-K) derived from Human TIE2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.

Applications:

WB

Background:

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Schooley K.A., Baum P.R. Oncogene 8:663-670(1993)
Dunham I. Nature 429:369-374(2004)
Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF