RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody

Catalog Number:

11753

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15418/P51812/Q15349/Q9UK32;
NCBI Gene#: 6195/6197/6196/27330;
NCBI Protein#: NP_002944.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 221/227/218/232(A-Y-S(p)-F-C) derived from Human RSK1/2/3/4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232.

Applications:

WB

Background:

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway.

Moller D.E., Am. J. Physiol. 266:C351-C359(1994).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF