PEK/PERK (Phospho-Thr982) Antibody

Catalog Number:

11751

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9NZJ5;
NCBI Gene#: 9451;
NCBI Protein#: NP_004827.4.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 982(R-H-T(p)-G-Q) derived from Human PEK/PERK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PEK/PERK only when phosphorylated at threonine 982.

Applications:

IHC

Background:

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1)

Shi Y., An J., J. Biol. Chem. 274:5723-5730(1999).
Sood R., Biochem. J. 346:281-293(2000).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF