MLK1/2 (Phospho-Thr312/266) Antibody

Catalog Number:

11744

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P80192/Q02779;
NCBI Gene#: 4293/4294;
NCBI Protein#: NP_001271159.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 312/266(A-G-T(p)-Y-A) derived from Human MLK1/2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266.

Applications:

IHC

Background:

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.

Durkin J.T., Biochemistry 43:16348-16355(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Dorow D.S.,Nature 446:153-158(2007).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF