MAP2K7 (Phospho-Thr275) Antibody

Catalog Number:

11743

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O14733;
NCBI Gene#: 5609;
NCBI Protein#: NP_660186.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 275(A-K-T(p)-R-S) derived from Human MAP2K7.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MAP2K7 only when phosphorylated at threonine 275.

Applications:

WB

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.

Wu Z.,Mol. Cell. Biol. 17:7407-7416(1997).
Lu X.,J. Biol. Chem. 272:24751-24754(1997).
The MGC Project Team;Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF