MAP2K3 (Phospho-Thr222) Antibody

Catalog Number:

11741

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P46734;
NCBI Gene#: 5606;
NCBI Protein#: NP_659731.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 222(A-K-T(p)-M-D) derived from Human MAP2K3 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.

Applications:

WB IHC

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

Derijard B., Science 267:682-685(1995).
Moriguchi T., J. Biol. Chem. 271:26981-26988(1996).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF