ILK (Phospho-Ser246) Antibody

Catalog Number:

11733

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13418;
NCBI Gene#: 3611;
NCBI Protein#: NP_001014794.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 246(I-F-S(p)-H-P) derived from Human ILK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of ILK only when phosphorylated at serine 246.

Applications:

IHC

Background:

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Hannigan G.E., Nature 379:91-96(1996).
Janji B., Oncogene 19:3069-3077(2000).
Tadic B., Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF