EPHA3/4/5 (Phospho-Tyr779/833) Antibody

Catalog Number:

11729

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P29320/54764/54756;
NCBI Gene#: 2042/2043;
NCBI Protein#: NP_004429.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 779/833 (E-A-Y(p)-T-T)/(A-A-Y(p)-T-T) derived from Human EPHA3/4/5.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of EPHA3/4/5 only when phosphorylated at tyrosine 779/833.

Applications:

IHC

Background:

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Wicks I.P., Proc. Natl. Acad. Sci. U.S.A. 89:1611-1615(1992).
Chiari R., Cancer Res. 60:4855-4863(2000).
Boyd A.W., J. Biol. Chem. 267:3262-3267(1992).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF