ALK (Phospho-Tyr1096) Antibody

Catalog Number:

11726

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9UM73;
NCBI Gene#: 238;
NCBI Protein#: NP_004295.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 1096 (P-N-Y(p)-C-F) derived from Human ALK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1096.

Applications:

IHC

Background:

The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain.

Morris S.W., Oncogene 14:2175-2188(1997).
Morris S.W., Oncogene 15:2883-2883(1997).
Iwahara T., Oncogene 14:439-449(1997).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF