ABL1 (Phospho-Thr735) Antibody

Catalog Number:

11725

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P00519;
NCBI Gene#: 25;
NCBI Protein#: NP_005148.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine735 (S-V-T(p)-L-P) derived from Human ABL1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735.

Applications:

WB

Background:

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

Fainstein E., Oncogene 4:1477-1481(1989).
Shtivelman E., Cell 47:277-284(1986).
Chissoe S.L., Genomics 27:67-82(1995).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF