HSF1 (Phospho-Thr142) Antibody

Catalog Number:

11715

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q00613;
NCBI Gene#: 3297;
NCBI Protein#: NP_005517.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 142(L-L-T(p)-D-V) derived from Human HSF1 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.

Applications:

IHC

Background:

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Schuetz T.J.,Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991)

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF