IGF2R (Phospho-Ser2409) Antibody

Catalog Number:

11708

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P11717;
NCBI Gene#: 3482;
NCBI Protein#: NP_000867.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 2409(Q-D-S(p)-E-D) derived from Human IGF2R.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of IGF2R only when phosphorylated at serine 2409.

Applications:

WB IHC

Background:

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.

Morgan D.O., Nature 329:301-307(1987).
Oshima A., J. Biol. Chem. 263:2553-2562(1988).
Killian J.K., Mamm. Genome 10:74-77(1999)

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF