WEE1 (Phospho-Ser642) Antibody

Catalog Number:

11706

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P30291;
NCBI Gene#: 7465;
NCBI Protein#: NP_003381.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.

Applications:

WB

Background:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Watanabe N., EMBO J. 14:1878-1891(1995).
Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).
Igarashi M., Nature 353:80-83(1991)

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF