Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody

Catalog Number:

11703

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P14598;
NCBI Gene#: 653361;
NCBI Protein#: NP_000256.4.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 304(R-S-S(p)-I-R) derived from Human Neutrophil Cytosol Factor 1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 304.

Applications:

WB

Background:

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A., J. Clin. Invest. 100:1907-1918(1997).
Chanock S.J., Blood Cells Mol. Dis. 26:37-46(2000)

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF