TGF β Receptor II (Phospho-Ser225/250) Antibody

Catalog Number:

11700

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P37173;
NCBI Gene#: 7048;
NCBI Protein#: NP_003233.4.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 225/250(D-R-S(p)-D-I) derived from Human TGF β Receptor II.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of TGF β Receptor II only when phosphorylated at serine 225/250.

Applications:

IHC

Background:

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis.

RC Tripathi, Invest. Ophthalmol. Vis. Sci., Jan 1993; 34: 260.
N Chegini, Endocrinology, Jul 1994; 135: 439.
GW Mulheron, Endocrinology, Dec 1992; 131: 2609

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF