PKC α (Phospho-Tyr658) Antibody

Catalog Number:

11684

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P17252;
NCBI Gene#: 5578;
NCBI Protein#: NP_002728.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 658(F-S-Y(p)-V-N) derived from Human PKC α.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PKC α only when phosphorylated at tyrosine 658.

Applications:

WB

Background:

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.

Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990).
McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991).
Gevaert K., Nat. Biotechnol. 21:566-569(2003)

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF