PEA-15 (Phospho-Ser104) Antibody

Catalog Number:

11676

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15121;
NCBI Gene#: 8682;
NCBI Protein#: NP_003759.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 104(I-P-S(p)-A-K) derived from Human PEA-15.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.

Applications:

WB IHC

Background:

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

Estelles A., J. Biol. Chem. 271:14800-14806(1996).
Condorelli G., EMBO J. 17:3858-3866(1998).
Wolford J.K., Gene 241:143-148(2000).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF