NMDAR1 (Phospho-Ser890) Antibody

Catalog Number:

11674

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q05586;
NCBI Gene#: 2902;
NCBI Protein#: NP_015566.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 890(A-S-S(p)-F-K) derived from Human NMDAR1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of NMDAR1 only when phosphorylated at serine 890.

Applications:

IHC IF

Background:

NMDA receptors are members of the ionotropic class of glutamate receptors, which also includes Kainate and AMPA receptors. NMDA receptors consist of NR1 subunits combined with one or more NR2 (A-D) or NR3 (A-B) subunits. The ligand-gated channel is permeable to cations including Ca2+, and at resting membrane potentials NMDA receptors are inactive due to a voltage-dependent blockade of the channel pore by Mg2+. NMDA receptor activation, which requires binding of glutamate and glycine, leads to an influx of Ca2+ into the postsynaptic region where it activates several signaling cascades, including pathways leading to the induction of long-term potentiation (LTP) and depression (LTD). NMDA receptors have a critical role in excitatory synaptic transmission and plasticity in the CNS. They govern a range of physiological conditions including neurological disorders caused by excitotoxic neuronal injury, psychiatric disorders and neuropathic pain syndromes.

Guang Bai, J. Biol. Chem., Jan 1998; 273: 1086.
Guang Bai, J. Biol. Chem., Mar 1995; 270: 7737.
Mai T. Dang, PNAS, Oct 2006; 103: 15254 - 15259.

References:

appl_detail:

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

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