CD32 (Phospho-Tyr292) Antibody

Catalog Number:

11672

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P31994 ;
NCBI Gene#: 2213;
NCBI Protein#: NP_003992.3.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 292 (I-T-Y(p)-S-L) derived from Human CD32.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CD32 only when phosphorylated at tyrosine 292.

Applications:

WB

Background:

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Stuart S.G., EMBO J. 8:3657-3666(1989).
Brooks D.G., J. Exp. Med. 170:1369-1385(1989).
Engelhardt W., Eur. J. Immunol. 20:1367-1377(1990).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF