Caspase 9 (Phospho-Thr125) Antibody

Catalog Number:

11649

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P55211;
NCBI Gene#: 842;
NCBI Protein#: NP_001220.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).

Applications:

WB IHC

Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Duan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999)

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF