PDHK1(Phospho-Thr338) Antibody

Catalog Number:

11596

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q15118
NCBI Protein: NP_001265478.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 338(Y-S-T(p)-A-P) derived from Human PDHK1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PDHK1 only when phosphorylated at threonine 338.

Applications:

WB

Background:

 Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

 

Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M.J. Biol. Chem. 270:28989-28994(1995)            

The MGC Project TeamGenome Res. 14:2121-2127(2004)

 

 

References:

appl_detail:

 Western blotting: 1:500~1:1000

Datasheet PDF