β-catenin（Phospho-Tyr333 ) β-catenin（Phospho-Tyr333 ) Antibody

Catalog Number:

11595

Amount:

100μg/100μl

Price:

$319

Swiss-Prot No:

Swiss-Prot: P07954
NCBI Protein: NP_000134.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Tyr333 of human β-catenin

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

β-catenin（Phospho-Tyr333)antibody detects endogenous levels of β-catenin only when phospholated at Tyrosine333 .

Applications:

Background:

In human cancer cells, that epidermal growth factor receptor (EGFR) activation induces translocation of PKM2 into the nucleus, where K433 of PKM2 binds to c-Src-phosphorylated Y333 of b-catenin. The transactivation of b-catenin is instrumental in EGFR promoted tumour cell proliferation and brain tumour development. Levels of b-catenin phosphorylation have been correlated with grades of glioma malignancy and prognosis [1] .

References:

[1] Yang W, Xia Y, Ji H, Zheng Y, Liang J, Huang W, Gao X, Aldape K, Lu Z. Nuclear PKM2 regulates β-catenin transactivation upon EGFR activation. Nature. 2011 Dec 1;480(7375):118-22. doi: 10.1038/nature10598.

appl_detail:

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