beta-catenin (Phospho-Ser715) Antibody

Catalog Number:

11594

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P35222
NCBI Gene ID: 1499

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Ser715 of human β-catenin

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

β-catenin（Phospho-Ser715)antibody detects endogenous levels of β-catenin only when phospholated at Serine715 .

Applications:

Background:

Aberrant activation of b-catenin in the nucleus has been implicated in a variety of human cancers. TRIM33 interacts with and ubiquitylates nuclear b-catenin. Moreover, protein kinase Cd, which directly phosphorylates b-catenin at Ser715, is required for the TRIM33–b-catenin interaction. In human glioblastoma specimens, endogenous TRIM33 levels are inversely correlated with b-catenin, we can abolish tumour cell proliferation and tumorigenesis by degrading nuclear b-catenin [1] .

References:

[1] Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S. Tumour suppressor TRIM33 targets nuclear β-catenin degradation. Nat Commun. 2015 Feb 2;6:6156. doi: 10.1038/ncomms7156.

appl_detail:

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