CK2a(phospho-Thr360/Ser362) Antibody

Catalog Number:

11572

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P68400
NCBI Protein: NP_001894.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2a.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of CK2 only when phosphorylated at thronine 360.

Applications:

WB

Background:

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The a and a' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001)
Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002)
Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005)
Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000)

References:

appl_detail:

Predicted MW: 42kd
Western blotting: 1:1000

Datasheet PDF