eIF2a(phospho-Ser49) Antibody

Catalog Number:

11511

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05198
NCBI Protein: NP_004085.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of eIF2a only when phosphorylated at Serine 49.

Applications:

WB IHC IF

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.

References:

appl_detail:

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF