IGF-1R(Phospho-Tyr1280) Antibody

Catalog Number:

11302

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P08069
NCBI Protein: NP_000866.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 1280 (S-F-Y(p)-Y-S) derived from Human IGF-1R

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of IGF-1R only when phosphorylated at tyrosine 1280.

Applications:

WB

Background:

This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. Ref.19 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, Ref.21 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093
Baserga, R. et al. (2000) Oncogene 19, 5574-5581
Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
O'Connor R, et al. Mol Cell Biol 1997 Jan; 17(1): 427-35

References:

appl_detail:

Predicted MW: 200 95kd
Western blotting: 1:500~1:1000

Datasheet PDF