Ephrin-B2(Phospho-Tyr316) Antibody

Catalog Number:

11188

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P52799
NCBI Protein: NP_004084.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y(p)-I-V) derived from Human Ephrin-B2

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 316.

Applications:

WB

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al.( 2006) Blood;107(6):2330-2338.
Noren NK, et al.( 2004) Proc Natl Acad Sci USA;101(15):5583-5588

References:

appl_detail:

Predicted MW: 37kd
Western blotting: 1:500~1:1000

Datasheet PDF