Cyclin D1 (Phospho-Thr286) Antibody

Catalog Number:

11968

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P24385;
NCBI Gene#: 595;
NCBI Protein#: NP_444284.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 286 (A-C-T(p)-P-T) derived from Human Cyclin D1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286.

Applications:

WB

Background:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40
Tagliati F, et al. (2006) Endocrinology 147, 3530-8
Zou Y, et al. (2004) J Biol Chem 279, 27790-8

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF