IKK- gamma (Phospho-Ser31) Antibody

Catalog Number:

11967

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9Y6K9;
NCBI Gene#: 8517;
NCBI Protein#: NP_001093326.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 31 (E-E-S(p)-P-L) derived from Human IKK-gamma.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IKK- gamma only when phosphorylated at serine 31.

Applications:

WB

Background:

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly.

Carter RS, Pennington KN, Ungurait BJ, Ballard DW (2003)J Biol Chem 278, 19642-8.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF