Cyclin H (Phospho-Thr315) Antibody

Catalog Number:

11689

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P51946;
NCBI Gene#: 902;
NCBI Protein#: NP_001230.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from Human Cyclin H .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.

Applications:

WB

Background:

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Maekelae T.P., Nature 371:254-257(1994).
Fisher R.P., Cell 78:713-724(1994).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF