PGK1 (Acetyl-Lys388) Antibody

Catalog Number:

11599

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P00558
NCBI Gene ID: 5230
NCBI mRNA#: NM_000291.3
NCBI Protein#: NP_000282.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around acetylation site of lysine 388(E-D-K(Acetyl)-V-S) derived from Human PGK1.

Purification:

Specificity/Sensitivity:

The antibody detects endogenous level of PGK1 only when acetylated at Lysine 388.

Applications:

WB IHC IP

Background:

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D).

References:

Xu Qian1,et al. Phosphoglycerate kinase 1 phosphorylates Beclin1 to induce autophagy.Mol Cell. 2017 March 02; 65(5): 917–931.e6. doi:10.1016/j.molcel.2017.01.027. Autho

appl_detail:

 WB dilution: 1:500~1:1000

 IHC dilution: 1:50~1:100

 IP dilution: 1:20~1:50

Datasheet PDF