Androgen Receptor (Phospho-Tyr363) Antibody

Catalog Number:

11761

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P10275;
NCBI Gene#: 367;
NCBI Protein#: NP_000035.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 363 (D-Y-Y(p)-N-F) derived from Human Androgen Receptor.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Androgen Receptor only when phosphorylated at tyrosine 363.

Applications:

WB

Background:

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Lubahn D.B., Mol. Endocrinol. 2:1265-1275(1988).
Chang C., Proc. Natl. Acad. Sci. U.S.A. 85:7211-7215(1988).
Tilley W.D., Proc. Natl. Acad. Sci. U.S.A. 86:327-331(1989).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF