Pim-1 (Phospho-Tyr309) Antibody

Catalog Number:

11677

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P11309;
NCBI Gene#: 5292;
NCBI Protein#: NP_001230115.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 309(H-R-Y(p)-H-G) derived from Human Pim-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.

Applications:

WB

Background:

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.

Selten G., Cell 46:603-611(1986).
Saris C.J., EMBO J. 10:655-664(1991).
Maita H., Eur. J. Biochem. 267:5168-5178(2000).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF