DLC1 (Ab-868) Antibody

Catalog Number:

21698

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q96QB1
NCBI Protein#: NP_872584.2.

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around aa.866~870 (R-N-S-S-S) derived from Human DLC1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Specificity/Sensitivity:

The antibody detects endogenous level of total DLC1 protein.

Applications:

WB

Background:

 Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.

 

1)protein DLC-1 on cell morphology and migration."Kim T.Y., Healy K.D., Der C.J., Sciaky N., Bang Y.J., Juliano R.L.J. Biol. Chem. 283:32762-32770(2008)

2)Kawai K., Iwamae Y., Yamaga M., Kiyota M., Ishii H., Hirata H., Homma Y., Yagisawa H.Genes Cells 14:227-241(2009)

3)Erlmann P., Schmid S., Horenkamp F.A., Geyer M., Pomorski T.G., Olayioye M.A.Mol. Biol. Cell 20:4400-4411(2009)

References:

appl_detail:

 Western blotting: 1:500~1:1000

Datasheet PDF