Bub3 Antibody

Catalog Number:

49949

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:O43684

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Recombinant protein corresponding to the N-terminus of human Bub3.

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB,ICC,IF,IP,IHC,FC

Background:

BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.

References:

appl_detail:

WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100

Datasheet PDF