eIF4A3 Antibody

Catalog Number:

49884

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P38919

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Recombinant protein within human eIF4A3 aa 1-150.

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB,ICC,IF,IHC,FC,IP

Background:

ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.

References:

appl_detail:

WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Datasheet PDF