NF-H Antibody

Catalog Number:

48320

Amount:

100μg

Price:

$529

Swiss-Prot No:

Swiss-Prot#:P12036

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

A neurofilament NF-H protein isolated from a cytoskeletal preparation from brain tissue homogenate of calf origin.

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF, IHC(P)

Background:

Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.

References:

appl_detail:

WB: 1:500-1:1000
IHC: 1:50-1:500

IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

Datasheet PDF