His-Tag Mouse Monoclonal Antibody (4E6)

Catalog Number:

Amount:

100μg

Price:

$529

Swiss-Prot No:

Form of Antibody:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purification:

Affinity purification using immunogen.

Specificity/Sensitivity:

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins..

Applications:

WB

Background:

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

References:

appl_detail:

Western blotting: 1:3000~1:10000

Datasheet PDF