DNA (cytosine-5) methyltransferase3A Polyclonal Antibody

Catalog Number:

42151

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9Y6K1

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human DNA (cytosine-5)-methyltransferase 3A protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total DNA (cytosine-5) methyltransferase3A polyclonal antibody.

Applications:

IHC

Background:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity

References:

[1]"Cloning, expression and chromosome locations of the human DNMT3 gene family."Xie S., Wang Z., Okano M., Nogami M., Li Y., He W.-W., Okumura K., Li E.Gene 236:87-95(1999) [2]"A novel Dnmt3a isoform produced from an alternative promoter localizes to eu

appl_detail:

Immunohistochemistry: 1:20 - 1:200

Datasheet PDF