CDK8 Polyclonal Antibody

Catalog Number:

42114

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P49336

Form of Antibody:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage/Stability:

Immunogen:

Recombinant human Cyclin-dependent kinase 8 protein(365-464aa)

Purification:

Antigen Affinity purified

Specificity/Sensitivity:

The antibody detects endogenous level of total CDK8 polyclonal antibody.

Applications:

IHC

Background:

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

References:

[1]Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamr

appl_detail:

Immunohistochemistry: 1:20 - 1:200

Datasheet PDF